Part:BBa_K2012013
Strong CheZ generator with a fusion GFP reporter
This generator contains promoter J23101 ,RBS from BBa_J61100,functionaal chemotaxis gene CheZ,reporter gene GFP from BBa_E0040 and a 18bp-GS linker between two functional domains. Promoters with different strength are also constructed as BBa_K2012014[1],BBa_K2012016[2]
We have proved the function of GFP and CheZ by observing it under the fluorescence microscope and transforming it into a cheZ lacking strain to process swarming assay seperately.
The image are as following.
Figure 1. CL1 control was observed under light field and fluorescence field.
.
Figure 2. E.coli with BBa_K2012013 was observed under light field and fluorescence field.
Figure 3. The swarming plate of E.coli K12 cheZ lacing strain strain with different strength expression of cheZ-GFP.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 715
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1362
| None |